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KMID : 1001020120100020041
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Korean Journal of Urological Oncology
2012 Volume.10 No. 2 p.41 ~ p.49
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Production of Cancer Diagnostic and Therapeutic mAb Using Plant and Insect Expression Systems
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Lee Jeong-Hwan
Kim Deuk-Su
Lee Jae-Hyouk
Myung Soon-Chul
Hwang Kyung-A
Choo Young-Kug
Ko Ki-Sung
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Abstract
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Therapeutic proteins can prevent or treat wide ranges of diseases from cancer and viral or bacterial infections. Production of the therapeutic proteins has been well established mainly in mammalian and bacterial cells including transgenic organisms by recombinant DNA techniques. Recent plant and insect biotechnology and advanced molecular immunology have established molecular biofarming system as an alternative way to produce recombinant pharmaceutical proteins such as immunotherapeutic monoclonal antibodies (mAb). The plants and insects as an expression system have several advantages, which include the lack of animal pathogenic contaminants, low cost of production, and ease of agricultural scale-up compared to other currently available systems. Thus, the paradigm of plant is being shifted as a food source to so-called plant bioreactor for production of therapeutic proteins. Currently, we have successfully developed a plant and insect cell expression system for production of anti-cancer and anti-virus monoclonal antibodies. The effective heterologous production system for recombinant therapeutics requires the appropriate expression machinery with optimal combination of transgene expression regulatory conditions such as control of transcriptional and post transcriptional events. In this paper, the possibility of targeting proteins to the ER and possibly storing them to the protein storage vacuoles with glycosylation modification are discussed in plant and insect cells.
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KEYWORD
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Plant, Insect cell, Therapeutic proteins
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